C1-INH Dpl

Product Description

Normal human serum was depleted of C1-Inhibitor (C1-INH) by immunoaffinity chromatography. The product is tested for the absence of C1-INH by double immunodiffusion. C1-INH is a regulator of the classical pathway of complement activation. The process of making C1-INH depleted serum (C1-INH Dpl) results in the activation of classical pathway components C2 and C4, hence their consumption. Thus C1-INH Dpl does not possess a functional classical pathway of complement.

            In the absence of C1-INH, a fully functional classical pathway of complement can be restored by adding purified C2 and C4 proteins at normal serum concentration to the C1-INH Dpl. These results suggest that besides C2 and C4 and in the absence of C1-INH all the other complement components necessary for classical pathway activation are present in C1-INH Dpl (CH50 assay) (Morgan, B.P. (2000); Dodds, A.W. and Sim, R.B. (1997)). The C1-INH Dpl reconstituted with C2 and C4 should not be subjected to a freeze/thaw cycle because in the absence of C1-INH, thawing of the C1-INH Dpl results in the activation of C2 and C4.

C1-INH Dpl is also tested for and certified to contain a functional alternative pathway indicating that all other complement components necessary for alternative pathway activation are present (Morgan, B.P. (2000); Dodds, A.W. and Sim, R.B. (1997)). 

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