Heat Inactivated Normal Human Serum (NEW)

Product Description

Heat inactivated normal human serum (HI-NHS) exhibits no complement activity via the classical and alternative pathways (CH50 and AP50). Normal human serum (neat NHS) tested for complement activity and certified to possess fully functional classical and alternative pathways of complement was used as the starting material to make HI-NHS. NHS was prepared from a large number of donors. Each unit of blood was collected without anticoagulants and each was individually tested for transfusion-transmissible diseases before pooling (see Precautions/Toxicity/Hazards below).  After coagulation, the liquid portion was separated by centrifugation.  The different units were pooled, filtered through a 0.22 µm filter and tested for complement activity via the classical and alternative pathways. The complement active NHS was then subjected to inactivation at 56^oC for 30 minutes, filtered through a 0.22 µm filter, aliqoted and frozen at -80^oC.

            All testing for complement activity was performed on the subsequently thawed samples to guarantee that the functional activity reported is what customers will receive when the samples are thawed.

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