VBSo is like the traditional buffer used in complement assays (GVBo) but it lacks the carrier protein gelatin often used in these assays. GVB may be replaced with VBS if gelatin is undesirable or may interfere with the assays. Ca++ and Mg++ may be added to VBSo to make VBS++ for classical pathway and lectin pathway assays. MgEGTA may be added to VBSo for alternative pathway assays. EDTA may be added to VBSo to prepare VBSE to inhibit complement activation. VBSo is also used for dilution of serum and other assay components in many complement assays especially in alternative pathway assays (Morgan, B.P. (2000; Dodds, A.W. and Sim, R.B. (1997)).
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