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VBS++ is like the traditional buffer used in complement assays (GVB++) but it lacks the carrier protein gelatin often used in these assays. GVB may be replaced with VBS if gelatin is undesirable or may interfere with the assays. The concentrations of metal ions are the traditional concentrations and these give maximal complement activation (see Buffer Components section below). Dilutions of serum and other assay components for CH50 assays may prepared in this VBS++ buffer (Morgan, B.P. (2000); Dodds, A.W. and Sim, R.B. (1997)).
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