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Name:                         Rabbit Anti-Human SC5b-9 Neoantigen

Catalog Number:       A227

Sizes Available:          250 µL

Concentration:           8 mg/mL purified IgG concentration (not affinity purified).         

Extinction Coeff.        A280 nm = 1.35 at 1.0 mg/mL

Form:                          Frozen liquid, clear solution.

Buffer:                        Phosphate-buffered saline, pH 7.2

Activity:                      1/4 dilution versus activated NHS assayed by immunodiffusion

                                    Not effective in Western Blots – SDS dissociates antigen.

                                    1/500 to 1/2000 effective concentrations in ELISA.

Preservative:              0.09 % sodium azide, filtered through 0.22 µm filter.

Storage:                      -20oC or below (long term), +4oC (short term, <30 days)

Source:                       Rabbits (Oryctolagus cuniculus) immunized with highly purified

                                    human complement protein.

Specificity:                  Monospecific for human SC5b-9 complex in plasma. No reaction

occurs with non-activated normal human serum or plasma.  Reacts with cell surface-bound C5b-9 complex.

Origin:                        Manufactured in the USA.

 

General Description

            Product is made from polyclonal antiserum from rabbits immunized with highly purified human complement protein.  Antisera is stringently adsorbed with normal complement proteins to be monospecific for SC5b-9 neoantigen.  Product sold is the purified IgG fraction. Rabbits are maintained in FDA certified facilities.

 

Physical Characteristics & Structure

            Antibodies present in the antisera are primarily IgG.

 

Applications

            Recognizes human SC5b-9 complex in complement-activated plasma.

            Reacts  with cell surface-bound C5b-9 complex after complement activation.

            Western Blots:  Not applicable.  Antibody recognizes SC5b-9 neoantigen which is

                         destroyed upon denaturation in SDS.

            ELISA:  Effective at dilutions 1/500 to 1/2000 depending on conditions.

            Immunodiffusion:  Effective against purified SC5b-9 as well as SC5b-9 in         

                        complement-activated NHS or plasma at ¼ dilution.

 

FOR RESEARCH USE ONLY.

NOT FOR HUMAN OR DRUG USE.

 

Complement Technology, Inc.

4801 Troup Hwy, Suite 701

Tyler, Texas  75703  USA

Phone:  903-581-8284

FAX:  903-581-0491

Email:  contactCTI@aol.com

Web:  www.ComplementTech.com